Effect of mechanical compression on the lumbar nerve root: localization and changes of intraradicular inflammatory cytokines, nitric oxide, and cyclooxygenase.

Abstract

STUDY DESIGN:

Investigation of intraradicular inflammation induced by mechanical compression.

OBJECTIVE:

To investigate the mechanism of nerve root pain, this study used a lumbar nerve root compression model.

SUMMARY OF BACKGROUND DATA:

The manifestation of pain at sites of inflammation has a close relationship with the release of mediators from macrophages. However, the mediators involved in inflammation of nerve roots as a result of mechanical compression remain almost unknown.

METHODS:

In this study, the seventh lumbar nerve root of dogs was compressed with a clip for 3 weeks to observe the changes caused by compression. Immunohistochemistry was performed using the avidin-biotin-peroxidase complex method to observe the changes of T cells (CD45) and macrophages (Mac-1) after compression. Antibodies against as interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), inducible nitric oxide synthase (i-NOS), and cyclooxygenase (COX)-1 and 2 were used to examine the localization and changes of these mediators caused by nerve root compression.

RESULTS:

In control animals, resident T cells were detected, but there were no macrophages. IL-1beta and COX-2 were positive in the Schwann cells and vascular endothelial cells, while COX-1 was detected in the vascular endothelial cells. However, no cells showed TNF-alpha or i-NOS positively. After nerve root compression, numerous T cells and macrophages appeared among the demyelinized nerve fibers. The macrophages were positive for IL-1beta, TNF-alpha, i-NOS, and COX-2.

CONCLUSION:

Inflammatory cytokines, NO, and COX-2 may be deeply involved in radiculitis caused by mechanical compression, and these mediators seem to be important in the manifestation of root pain.

• Authors: Shigeru Kobayashi, Hisatoshi Baba, Kenzo Uchida, Yasuo Kokubo, Chikara Kubota, Syuuich Yamada, Yoshihiko Suzuki, Hidezo Yoshizawa
• Journal: Spine (Spine (Phila Pa 1976)) Vol. 30 Issue 15 Pg. 1699-705 (Aug 01 2005) ISSN: 1528-1159 [Electronic] United States
• PMID: 16094269 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cytokines
• Nitric Oxide
• Prostaglandin-Endoperoxide Synthases

Topics

• Animals
• Cytokines (analysis, biosynthesis)
• Dogs
• Lumbar Vertebrae (chemistry, metabolism, pathology)
• Nerve Compression Syndromes (metabolism, pathology)
• Nitric Oxide (analysis, biosynthesis)
• Prostaglandin-Endoperoxide Synthases (analysis, biosynthesis)
• Radiculopathy (metabolism, pathology)
• Spinal Nerve Roots (chemistry, metabolism, pathology)
• Stress, Mechanical