Abstract | BACKGROUND: An impaired production of interleukin (IL)-12 and T cell interferon-gamma (IFN-gamma) of in vitro stimulated monocytes has been discussed as a pathogenic factor in Whipple's disease (WD). It is unclear whether this defect of cellular immunity is translated to the humoral immune system and to serum correlates. METHODS: RESULTS: We observed a highly significant reduction of IL-12p40 levels (patients, 0.18+/-0.05 ng/ml (mean+/-SEM); controls, 3.19+/-0.39 ng/ml; p<0.01) in all stages of disease activity, whereas the concentration of IL-12p70 was comparable with controls. Furthermore, we observed a slight decrease in tumour necrosis factor alpha ( TNF-alpha) concentrations in the serum of patients (patients, 6.36+/-0.90 pg/ml; controls, 10.5+/-1.23 pg/ml; p<0,05). The levels of other cytokines such as IFN-gamma, IL-2, IL-13 and transforming growth factor beta, as well as soluble cell adhesion molecules lymphocyte function-associated antigen 3 and intercellular adhesion molecule 1, were not significantly different compared with controls. Levels of immunoglobulin G2 ( IgG2) measured in the serum of WD patients were below normal in 24 of 29 patients and were even below the 95% confidence interval in 10 patients. CONCLUSION: Our data demonstrate a persistent defect of the cellular immune response with decreased serum concentrations of IL-12p40 and TNF-alpha and decreased IgG2 levels in a large group of WD patients. These data support as in vivo finding the results obtained in previous investigations with stimulated monocytes/lymphocytes. The isolated decrease in IL-12p40 may hint at possible defects in the IL-12/IFN-gamma promoter system.
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Authors | A Kalt, T Schneider, S Ring, J Hoffmann, M Zeitz, A Stallmach, D H Persing, T Marth |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 21
Issue 2
Pg. 114-20
(Mar 2006)
ISSN: 0179-1958 [Print] Germany |
PMID | 15875203
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Immunoglobulin G
- Interleukin-12 Subunit p40
- Tumor Necrosis Factor-alpha
- Interleukin-12
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Topics |
- Biomarkers
(blood)
- Disease Progression
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunoglobulin G
(blood)
- Interleukin-12
(blood)
- Interleukin-12 Subunit p40
(blood)
- Male
- Middle Aged
- Severity of Illness Index
- Tumor Necrosis Factor-alpha
(blood)
- Whipple Disease
(blood)
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