Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF.

Abstract

To investigate the intra- and extracellular levels of various cytokines and chemokines in CSF in chronic inflammatory demyelinating polyneuropathy (CIDP) and vasculitic neuropathy (VN), 16 cytokines, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, IFN-gamma, TNF-alpha, G-CSF, MCP-1 and MIP-1beta, were measured in CSF supernatant by a multiplexed fluorescent bead-based immunoassay and intracellular production of IFN-gamma and IL-4 in CSF CD4+ T cells were simultaneously measured by flow cytometry in 14 patients with CIDP, 8 patients with VN and 25 patients with other noninflammatory neurologic diseases (OND). In the CSF supernatant, a significant increase of IL-17, IL-8 and IL-6, and a significant decrease of IL-4, IL-5 and IL-7 levels were detected in pretreated CIDP as compared with OND. A significant increase of IL-6, IL-8 and IL-10 levels was found in pretreated VN. Both IL-17 and IL-8 levels correlated strongly with CSF protein levels in CIDP, although the correlation of IL-6 levels was weak. In CSF CD4+ T cells, IFN-gamma+ IL-4- cell percentages were markedly elevated in CIDP compared with OND, but not in VN, resulting in a significant increase of intracellular IFN-gamma/IL-4 ratio in CIDP, even in the absence of CSF pleocytosis. The nonresponders to intravenous immunoglobulins (IVIGs) showed a significantly lower IFN-gamma- IL-4+ CD4+ T cell percentage, and tended to have a higher intracellular IFN-gamma/IL-4 ratio than the responders in CSF. Marked upregulation of Th1 cytokine, IL-17, and downregulation of Th2 cytokines, together with infiltration of IFN-gamma-producing CD4+ T cells are useful markers for CIDP, while several Th2 cytokines are upregulated in VN in CSF.

• Authors: Feng-Jun Mei, Takaaki Ishizu, Hiroyuki Murai, Manabu Osoegawa, Motozumi Minohara, Kun-Nan Zhang, Jun-ichi Kira
• Journal: Journal of the neurological sciences (J Neurol Sci) Vol. 228 Issue 1 Pg. 75-85 (Jan 15 2005) ISSN: 0022-510X [Print] Netherlands
• PMID: 15607214 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokines
• Cytokines

Topics

• Adult
• Aged
• Aged, 80 and over
• CD4-Positive T-Lymphocytes (metabolism)
• Chemokines (cerebrospinal fluid)
• Cytokines (cerebrospinal fluid)
• Extracellular Space (metabolism)
• Female
• Flow Cytometry (methods)
• Humans
• Immunoassay (methods)
• Linear Models
• Male
• Middle Aged
• Polyradiculoneuropathy, Chronic Inflammatory Demyelinating (cerebrospinal fluid, pathology)
• Th1 Cells (metabolism)
• Th2 Cells (metabolism)
• Vasculitis, Central Nervous System (cerebrospinal fluid, pathology)