Influence of postoperative acute-phase response on angiogenesis and tumor growth: open vs. laparoscopic-assisted surgery in mice.

Abstract

Inflammatory responses and tumor growth are increased after laparotomy compared with laparoscopy in some animal models. Proinflammatory cytokines interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) upregulate the expression of vascular endothelial growth factor (VEGF). Our aim was to investigate the influence of postoperative inflammatory responses on angiogenesis and tumor growth. 5 x 10(6) B51LiM cells were injected into the cecal wall of Balb/c mice. After 2 weeks, the animals were randomized into the following three groups: open cecectomy (OC), CO(2)-laparoscopic-assisted cecectomy (LC), and helium-laparoscopic-assisted cecectomy (LH). On postoperative day 12, the mice were killed. Tumor load scores and weight were significantly greater after laparotomy than after laparoscopy. Serum IL-6 levels 6 hours after surgery (OC: 4157+/-1297 pg/ml vs. LC: 2514+/-1417 pg/ml vs. LH: 2255+/-1714 pg/ml) and VEGF levels on postoperative day 12 (OC: 231+/-125 pg/ml vs. LC: 45+/-9 pg/ml vs. LH: 49+/-8 pg/ml), measured by enzyme-linked immunosorbent assay, were significantly higher in the laparotomy group. Microvessel density was also significantly higher in the OC group (OC: 34.3+/-11.5 vs. LC: 15.5+/-12.5 vs. LH: 18.5+/-11.9). There was a positive correlation between IL-6 and VEGF postoperative serum levels (rho=0.67; P<0.001). We concluded that increased systemic levels of proinflammatory cytokines and VEGF are associated with increased angiogenesis and tumor growth after laparotomy compared to laparoscopy in mice.

• Authors: Miguel Pera, Heidi Nelson, S Vincent Rajkumar, Tonia M Young-Fadok, Lawrence J Burgart
• Journal: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (J Gastrointest Surg) 2003 Sep-Oct Vol. 7 Issue 6 Pg. 783-90 ISSN: 1091-255X [Print] United States
• PMID: 13129557 (Publication Type: Clinical Trial, Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1
• Interleukin-6
• Vascular Endothelial Growth Factor A

Topics

• Acute-Phase Reaction (blood, etiology)
• Animals
• Cecal Neoplasms (blood, pathology, surgery)
• Chi-Square Distribution
• Enzyme-Linked Immunosorbent Assay
• Immunohistochemistry
• Interleukin-1 (blood)
• Interleukin-6 (blood)
• Laparoscopy (adverse effects, methods)
• Mice
• Mice, Inbred BALB C
• Neoplasm Metastasis
• Neoplasm Recurrence, Local
• Neovascularization, Pathologic (blood)
• Statistics, Nonparametric
• Tumor Cells, Cultured
• Vascular Endothelial Growth Factor A (blood)