Abstract | BACKGROUND: METHODS:
mRNA expression of cytokines and iNOS in inflamed and healthy gingival tissue was determined using reverse transcription-polymerase chain reaction (RT-PCR), and the relationship between their profiles and the detection of specific bacteria was analyzed. RESULTS: The relative expression of interleukin (IL)-6 and iNOS mRNAs in periodontal lesions was significantly higher than those in healthy individuals. IL-6 mRNA expression was also significantly higher at bleeding on probing (BOP)-positive sites than at BOP-negative sites. The expressions of IL-1alpha and IL-8 increased, but IL-10 expression decreased at sites where A. actinomycetemcomitans was detected. We found no correlation between the expression of cytokine and iNOS mRNA and infection by P. gingivalis. CONCLUSIONS: The expression of IL-6 may reflect inflammation in gingival tissue, and iNOS may be involved in the inflammatory process in periodontitis. The presence of A. actinomycetemcomitans or P. gingivalis might relate to the different cytokine profiles of IL-1alpha, IL-8, and IL-10.
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Authors | M Hirose, K Ishihara, A Saito, T Nakagawa, S Yamada, K Okuda |
Journal | Journal of periodontology
(J Periodontol)
Vol. 72
Issue 5
Pg. 590-7
(May 2001)
ISSN: 0022-3492 [Print] United States |
PMID | 11394393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokines
- Cytokines
- Inflammation Mediators
- Interleukin-1
- Interleukin-6
- Interleukin-8
- RNA, Messenger
- Interleukin-10
- Nitric Oxide Synthase
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Topics |
- Actinobacillus Infections
(enzymology, immunology)
- Adolescent
- Adult
- Aged
- Aggregatibacter actinomycetemcomitans
(physiology)
- Bacteroidaceae Infections
(enzymology, immunology)
- Chemokines
(analysis, genetics)
- Child
- Cytokines
(analysis, genetics)
- Female
- Gene Expression Regulation
- Gene Expression Regulation, Enzymologic
- Gingival Hemorrhage
(enzymology, immunology)
- Gingivitis
(enzymology, immunology, metabolism, microbiology)
- Humans
- Inflammation Mediators
(analysis)
- Interleukin-1
(analysis, genetics)
- Interleukin-10
(analysis, genetics)
- Interleukin-6
(analysis, genetics)
- Interleukin-8
(analysis, genetics)
- Male
- Middle Aged
- Nitric Oxide Synthase
(analysis, genetics)
- Periodontal Pocket
(enzymology, immunology)
- Periodontitis
(enzymology, immunology, metabolism, microbiology)
- Porphyromonas gingivalis
(physiology)
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Statistics as Topic
- Statistics, Nonparametric
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