Cytokines in aqueous humour and serum before and after corneal transplantation and during rejection.

Abstract

Cytokine profiles in aqueous humour were studied in relation to corneal disease and subsequent corneal graft survival or rejection. Cytokine levels in samples obtained from eyes with clear grafts (n = 59) were all within the normal range. At the time of penetrating keratoplasty (n = 146), intraocular levels of IL-6 were increased in 38% (50/131), most markedly in eyes with previous allograft failure or herpetic stromal keratitis. The level of IL-10 was increased in 1 eye (n = 144) and of IL-4 and IFN-gamma in none. During rejection (n = 10), the levels of IL-6 in aqueous humour were increased in 75% (3/4), of IL-10 in 50% (3/6), of IL-4 in none (0/4) and of IFN-gamma in 40% (2/5). In conclusion, the levels of total protein and IL-6 were increased prior to penetrating keratoplasty in eyes with previous inflammation. These results could however not predict the final outcome of the graft. Increased intraocular levels of IL-6, IL-10 and IFN-gamma were observed during rejection.

• Authors: B E van Gelderen, A Van Der Lelij, R Peek, L Broersma, W F Treffers, J M Ruijter, R van Der Gaag
• Journal: Ophthalmic research (Ophthalmic Res) 2000 Jul-Aug Vol. 32 Issue 4 Pg. 157-64 ISSN: 0030-3747 [Print] Switzerland
• PMID: 10828736 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 S. Karger AG, Basel

Chemical References

• Biomarkers
• Cytokines

Topics

• Adult
• Aged
• Aged, 80 and over
• Aqueous Humor (metabolism)
• Biomarkers
• Corneal Diseases (surgery)
• Cytokines (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Female
• Graft Rejection (immunology, metabolism)
• Humans
• Keratitis, Herpetic (metabolism)
• Keratoplasty, Penetrating
• Male
• Middle Aged
• Prognosis
• Retrospective Studies