Comparison of the abilities of human parathyroid hormone (hPTH)-(1-34) and [Leu27]-cyclo(Glu22-Lys26)-hPTH-(1-31)NH2 to stimulate femoral trabecular bone growth in ovariectomized rats.

Abstract

hPTH-(1-31)NH2, so far the smallest of the potently anabolic N-terminal fragments of the human parathyroid hormone, stimulates trabecular growth in the distal femurs of ovariectomized (OVX) rats as strongly as hPTH-(1-34) when injected at a high daily dose such as 1 nmol/100 g of body weight, but it is only about 70% as effective as hPTH-(1-34) when injected at the suboptimal 0.6 nmol/100 g of body weight. A lactam derivative of hPTH-(1-31)-NH2, [Leu27]-cyclo(Glu22-Lys26)-hPTH-(1-31)NH2, is a much more effective stimulator of adenylyl cyclase in ROS 17/2 rat osteoblast-like cells and a significantly more effective stimulator of femoral trabecular growth in OVX rats than hPTH-(1-31)NH2. We have now shown that [Leu27]-cyclo(Glu22-Lys26)-hPTH-(1-31)NH2 prevents the OVX-induced loss of femoral trabeculae significantly more effectively than hPTH-(1-34) and stimulates the thickening of the trabeculae remaining in severely depleted femoral trabecular bone of OVX rats as effectively as hPTH-(1-34) when injected at 0.6 nmol/100 g of body weight.

• Authors: J F Whitfield, P Morley, G Willick, S MacLean, V Ross, R J Isaacs, J R Barbier
• Journal: Calcified tissue international (Calcif Tissue Int) Vol. 63 Issue 5 Pg. 423-8 (Nov 1998) ISSN: 0171-967X [Print] United States
• PMID: 9799828 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Parathyroid Hormone
• Peptide Fragments
• parathyroid hormone (1-31)-NH2, leucyl(27)-cyclo(glutamyl(22)-lysyl(26))-
• Teriparatide
• Adenylyl Cyclases

Topics

• Adenylyl Cyclases (metabolism)
• Animals
• Bone Density (drug effects, physiology)
• Bone Development (drug effects, physiology)
• Cell Line
• Female
• Humans
• Osteoblasts (enzymology)
• Ovariectomy
• Parathyroid Hormone (pharmacology)
• Peptide Fragments (pharmacology)
• Rats
• Teriparatide (pharmacology)