Abstract | OBJECTIVE: DESIGN: We studied sera from nine children with OM, three with associated neuroblastoma and six with a prodromal viral illness. Control subjects (n = 77) included four children with neuroblastoma but not OM, 32 children with other neurologic disorders, and 41 with nonneurologic illnesses. We studied the neural antigenic targets of serum IgM and IgG autoantibodies by the following methods: (1) immunostaining of human cerebellar sections and peripheral nerve, and (2) Western blot analysis with human brain fractions including white matter, gray matter, and cerebellar Purkinje cells and nuclei. RESULTS: Sera from all nine children with OM had IgM and IgG binding to the cytoplasm of cerebellar Purkinje cells and to some axons in white matter. In peripheral nerve, IgM and IgG from all nine OM sera bound to large and small axons. Western blot analysis showed a distinctive pattern of binding to several neural proteins, including a 210 kd antigen identified as the high molecular weight subunit of neurofilament. No control serum showed a similar pattern of reactivity. CONCLUSION:
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Authors | A M Connolly, A Pestronk, S Mehta, M R Pranzatelli 3rd, M J Noetzel |
Journal | The Journal of pediatrics
(J Pediatr)
Vol. 130
Issue 6
Pg. 878-84
(Jun 1997)
ISSN: 0022-3476 [Print] United States |
PMID | 9202608
(Publication Type: Case Reports, Journal Article)
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Chemical References |
- Antigens
- Autoantibodies
- Immunoglobulin G
- Immunoglobulin M
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Topics |
- Adolescent
- Antigens
(analysis)
- Autoantibodies
(blood)
- Binding Sites
- Blotting, Western
- Brain
(metabolism)
- Cerebellum
(metabolism)
- Child, Preschool
- Female
- Humans
- Immunoglobulin G
(blood, metabolism)
- Immunoglobulin M
(blood, metabolism)
- Infant
- Infant, Newborn
- Male
- Myoclonus
(etiology, immunology)
- Neuroblastoma
(complications)
- Ocular Motility Disorders
(etiology, immunology)
- Paraneoplastic Syndromes
(complications)
- Purkinje Cells
(metabolism)
- Syndrome
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