Dissemination of MHV4 (strain JHM) infection does not require specific coronavirus receptors.

Abstract

In this report, we demonstrate the syncytial spread of MHV4 (strain JHM) infection through non-murine cell cultures which lack a specific MHV4 receptor and are therefore resistant to infection by free virions. This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay. Receptor-independent syncytium formation induced by cells expressing the MHV4 spike (S) from recombinant vaccinia viruses (VV) indicated that spread was mediated by this coronavirus glycoprotein. We conclude that the S protein of MHV4 is so potently fusogenic that it does not require prior binding to a virus-specific surface receptor to induce fusion of closely-opposed plasma membranes.

• Authors: T M Gallagher, M J Buchmeier, S Perlman
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 342 Pg. 279-84 ( 1993) ISSN: 0065-2598 [Print] United States
• PMID: 8209743 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Membrane Glycoproteins
• Receptors, Coronavirus
• Receptors, Virus
• Recombinant Fusion Proteins
• Spike Glycoprotein, Coronavirus
• Viral Envelope Proteins
• spike glycoprotein, SARS-CoV
• spike protein, mouse hepatitis virus

Topics

• Animals
• Astrocytoma
• Cell Fusion
• Cell Line (microbiology)
• Cricetinae
• Cytopathogenic Effect, Viral
• Genetic Vectors
• Kidney
• Membrane Glycoproteins (chemistry, physiology)
• Mesocricetus
• Murine hepatitis virus (physiology)
• Rats
• Receptors, Coronavirus
• Receptors, Virus (deficiency, physiology)
• Recombinant Fusion Proteins (metabolism)
• Species Specificity
• Spike Glycoprotein, Coronavirus
• Tumor Cells, Cultured (microbiology)
• Viral Envelope Proteins (chemistry, physiology)
• Virus Replication