A chromogenic assay for coagulation factor VII: analytical performance characteristics and application in several diseases.
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| Abstract |
A chromogenic assay for coagulation factor VII has been adapted for use on a Cobas Bio centrifugal analyzer. The interassay coefficients of variation ranged from 4.1% to 14%. There was no effect on the factor VII amidolytic activity after freezing and thawing of the samples. The test was linear up to 200% factor VII. The correlation with a chronometric method for prothrombin time was acceptable (r = 0.97, y = 1.05 x + 5.5). The reference ranges for males (54%-138%) and females (56%-200%) were significantly different. The factor VII amidolytic activities were significantly elevated in males with non-insulin-dependent diabetes mellitus and in male hypertensives. No statistically significant increase was observed for female patients rather a decrease in females with insulin-dependent diabetes mellitus. Only in the male hypertensive patients and in the male patients with non-insulin-dependent diabetes mellitus were noticeable proportions of increased values seen (39.4% and 12.0%). Nevertheless, the availability of a precise, rapid and easy to perform test for the determination of factor VII might be useful in the study of the role of this factor in the development of cardiovascular disease.
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| Authors | J W van Wersch |
| Journal | International journal of clinical & laboratory research (Int J Clin Lab Res) Vol. 23 Issue 4 Pg. 221-4 ( 1993) ISSN: 0940-5437 [Print] Germany |
| PMID | 8123879 (Publication Type: Journal Article) |
| Chemical References |
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