Abstract |
1. The neurohypophysial osmoregulatory hormones of the African toad Bufo regularis, a species adapted to estivate under dry and hot conditions, have been investigated. Vasotocin and hydrin 2 (vasotocinyl-Gly) have been identified by their retention times in high-pressure reverse-phase liquid chromatography and coelution with synthetic peptides, their pharmacological properties ( vasotocin) and microsequencing. 2. Vasotocin-associated neurophysin (MSEL- neurophysin type) has been characterized by its N-terminal amino acid sequence. 3. In toads subjected to dehydration by evaporation (20% weight loss) or to osmotic stress by immersion in 2% NaCl for 3 hr (6% weight loss), the molar ratio hydrin 2/ vasotocin (about 2:1) remained similar to the one observed in control animals. 4. In toads exposed to saline solution, there was a large decrease (roughly 30%) in the amounts of both hormones in the neuro-hypophysis. Environmental conditions for distinct secretions of vasotocin and hydrin 2 remain to be found.
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Authors | J Chauvet, Y Ouedraogo, G Michel, R Acher |
Journal | Comparative biochemistry and physiology. Comparative physiology
(Comp Biochem Physiol Comp Physiol)
Vol. 104
Issue 3
Pg. 497-502
(Mar 1993)
England |
PMID | 8097151
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neurophysins
- Protein Precursors
- Arginine Vasopressin
- hydrin 2
- Oxytocin
- Vasotocin
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Topics |
- Amino Acid Sequence
- Animals
- Arginine Vasopressin
(metabolism)
- Biological Assay
- Bufonidae
- Chromatography, High Pressure Liquid
- Dehydration
(physiopathology)
- Environment
- Female
- In Vitro Techniques
- Molecular Sequence Data
- Neurophysins
(metabolism)
- Osmolar Concentration
- Oxytocin
- Pituitary Gland, Posterior
(metabolism)
- Pregnancy
- Protein Precursors
(metabolism)
- Rats
- Spectrophotometry, Ultraviolet
- Vasotocin
(analogs & derivatives, metabolism)
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