Monoclonal B cells and restricted oligoclonal T cells in T-cell-rich B-cell lymphoma.

Abstract

Immunophenotyping of lymphoma using paraffin-embedded lymphoid tissue is useful in identifying the large neoplastic B cells in T-cell-rich B-cell lymphoma (TRBL), but does not succeed in deciding clonality. We studied six cases to determine the clonal population of B and T cells of TRBL. Immunohistochemistry on frozen and paraffin-embedded material showed that the cellular population in all six cases consisted mainly of T cells; fewer than ten percent of the cells stained as B cells. However, in all cases, monoclonality of the immunoglobulin was helpful for diagnosing the B-cell neoplasia. Southern blot-yielded genetic analysis showed monoclonality of B cells in three cases, but no evidence of clonality in the T cells. Moreover, gene monoclonality has been detected in all cases examined by polymerase chain reaction, using the primers for the V and J regions of the immunoglobulin heavy chain gene. For T cells, the D and J regions of the T-cell receptor (TCR) beta chain showed the same patterns of oligoclonal bands in all cells, and the V and J regions of the TCR gamma chain showed the same bands in all. The expression of TCR V beta families was polyclonal but restricted.

• Authors: K Ohshima, Y Masuda, M Kikuchi, Y Sumiyoshi, S Kobari, S Yoneda, M Takeshita, N Kimura
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 190 Issue 1 Pg. 15-24 (Jan 1994) ISSN: 0344-0338 [Print] Germany
• PMID: 8065986 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Molecular Probes

Topics

• Aged
• B-Lymphocytes (pathology)
• Base Sequence
• Blotting, Southern
• Clone Cells
• DNA, Neoplasm (analysis)
• Female
• Humans
• Immunohistochemistry
• Lymphoma, B-Cell (genetics, metabolism, pathology)
• Male
• Middle Aged
• Molecular Probes (genetics)
• Molecular Sequence Data
• Polymerase Chain Reaction
• T-Lymphocytes (pathology)
• Transcription, Genetic