Abstract |
To clarify which immunological factors were more effective in preventing influenza virus infection, we measured immunological parameters induced by vaccination and infection in vivo and in vitro. Healthy adult subjects (n = 128) were divided into vaccinated (n = 85) and untreated (n = 43) groups. Eighty-five were vaccinated intranasally with a trivalent cold-adapted recombinant influenza virus vaccine containing type A (H1N1 and H3N2) and B viruses. Subjects were mostly seropositive before vaccination. In 29 (80.6%) of the 36 examinees showing a prevaccination HI antibody titre of less than 1:128, the titre increased more than four times after vaccination. On the other hand, an increase of more than four times was found in four (8.2%) of the 49 individuals who had shown a prevaccination titre of more than 1:128. The IgA antibody was negligibly detected in the nasal wash specimens before vaccination, and was induced by vaccination in some cases. Lymphocyte proliferation and interleukin 2 (IL-2) production in cultured lymphocytes of the same subjects stimulated by H1N1 virus in vitro were correlated with the HI antibody titre. However, the interferon gamma (IFN-gamma) production was low before vaccination, regardless of the HI antibody titre, and showed a significant increase after vaccination. It was suggested that local IgA response and IFN-gamma production play important roles in the prevention of influenza. Since there was the outbreak of influenza A (H1N1) in Kochi Prefecture after completion of blood samples 6-8 weeks after the second vaccination, we examined the above hypothesis.(ABSTRACT TRUNCATED AT 250 WORDS)
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Authors | T Tomoda, H Morita, T Kurashige, H F Maassab |
Journal | Vaccine
(Vaccine)
Vol. 13
Issue 2
Pg. 185-90
(Feb 1995)
ISSN: 0264-410X [Print] Netherlands |
PMID | 7625114
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin A
- Influenza Vaccines
- Interleukin-2
- Vaccines, Attenuated
- Vaccines, Synthetic
- Interferon-gamma
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Topics |
- Administration, Intranasal
- Adult
- Disease Outbreaks
(prevention & control)
- Female
- Humans
- Immunoglobulin A
(biosynthesis)
- Influenza A virus
(immunology)
- Influenza B virus
(immunology)
- Influenza Vaccines
(administration & dosage, immunology, therapeutic use)
- Influenza, Human
(epidemiology, immunology, prevention & control)
- Interferon-gamma
(biosynthesis)
- Interleukin-2
(biosynthesis)
- Lymphocyte Activation
(drug effects)
- Male
- Surveys and Questionnaires
- Vaccines, Attenuated
(administration & dosage, immunology, therapeutic use)
- Vaccines, Synthetic
(administration & dosage, immunology, therapeutic use)
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