Release of dopamine, noradrenaline and dopamine B-hydroxylase from mouse neuroblastoma.

Abstract

The murine C1300 neuroblastoma tumor was found to secrete dopamine, noradrenaline and dopamine B-hydroxylase into the circulation of tumor-bearing A/J mice. The plasma levels of dopamine, noradrenaline and dopamine B-hydroxylase increased with the size of the tumor, and the increase in noradrenaline paralleled the increase in dopamine B-hydroxylase (r = 0.86). The vesicular storage of dopamine and noradrenaline in the tumor was evidenced by a decrease of the tissue content of dopamine and noradrenaline 24 hours after the administration of reserpine (5 micrograms/g) respectively to 17.6% and 7.8% of control values. A similar observation could be made for the levels of dopamine and noradrenaline in the plasma of reserpinized C1300 mice. The total activity of dopamine B-hydroxylase in the tumor and in plasma was unaffected by the reserpine treatment. Chronic administration of 6-hydroxydopamine (100 micrograms/g for 8 days) had no effect on the tissue contents of dopamine, noradrenaline or dopamine B-hydroxylase. The release of catecholamines and dopamine B-hydroxylase from the C1300 neuroblastoma was studied in vitro on superfused tumor slices. Stimulation of these slices with 56 mM KC1 or with 5.10(-5) M tyramine failed to induce the release of endogenous dopamine, noradrenaline or dopamine B-hydroxylase above the basal outflow levels. These results are suggestive for a non-exocytotic release of catecholamines and dopamine B-hydroxylase from the neuroblastoma tumor.

• Authors: N H Fraeyman, F H De Smet, E J Van de Velde, E J Moerman, A F De Schaepdryver
• Journal: Life sciences (Life Sci) Vol. 35 Issue 6 Pg. 619-29 (Aug 06 1984) ISSN: 0024-3205 [Print] Netherlands
• PMID: 6431216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxydopamines
• Potassium Chloride
• Reserpine
• Oxidopamine
• Dopamine beta-Hydroxylase
• Dopamine
• Norepinephrine

Topics

• Animals
• Dopamine (metabolism)
• Dopamine beta-Hydroxylase (metabolism)
• Hydroxydopamines (pharmacology)
• Male
• Mice
• Mice, Inbred A
• Neuroblastoma (metabolism)
• Norepinephrine (metabolism)
• Oxidopamine
• Potassium Chloride (pharmacology)
• Reserpine (pharmacology)
• Time Factors