Characterization of NADPH-cytochrome P-450 reductase in a mouse hepatoma cell line.

Abstract

NADPH-cytochrome c reductase in Hepa-1 cells was induced 2-fold by phenobarbital, but was not induced by benz[a]anthracene or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The apparent Km of the enzyme for NADPH was 0.57 microM; the activity was inhibitable by NADP; and segregated primarily to the microsomal fraction. Cytoplasm of Hepa-1 cells bound antibody to rabbit cytochrome P-450 reductase. 3T3 cells, which possessed one sixth of the cytochrome c reductase activity of Hepa-1 cells, bound correspondingly less cytochrome P-450 reductase antibody. This supports the notion that cytochrome P-450 reductase was responsible for the cytochrome c reductase activity that was measured.

• Authors: G S Duthu, M S Nestor, J A Berliner, R M Philpot, O Hankinson
• Journal: Cancer letters (Cancer Lett) Vol. 18 Issue 3 Pg. 237-43 (Apr 1983) ISSN: 0304-3835 [Print] Ireland
• PMID: 6303557 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Benz(a)Anthracenes
• Polychlorinated Dibenzodioxins
• 1,3,7,9-tetrachlorodibenzo-p-dioxin
• benz(a)anthracene
• Aryl Hydrocarbon Hydroxylases
• NADPH-Ferrihemoprotein Reductase
• Phenobarbital

Topics

• Animals
• Aryl Hydrocarbon Hydroxylases (metabolism)
• Benz(a)Anthracenes (pharmacology)
• Carcinoma, Hepatocellular (enzymology)
• Cell Line
• Enzyme Induction
• Fluorescent Antibody Technique
• Kinetics
• Liver Neoplasms (enzymology)
• Mice
• NADPH-Ferrihemoprotein Reductase (metabolism)
• Neoplasms, Experimental (enzymology)
• Phenobarbital (pharmacology)
• Polychlorinated Dibenzodioxins (analogs & derivatives, pharmacology)
• Rats