Abstract |
The DNase I (EC 3.1.21.1) sensitivities of the simian virus 40 (SV40) genome, the pBR322 genome, and the herpes simplex virus type 1 thymidine kinase (HSV-1 tk) gene have been compared in teratocarcinoma-derived stem (12-1) and differentiated (12-1a) cell lines established by transfection of thymidine kinase ( ATP: thymidine 5'- phosphotransferase, EC 2.7.1.21)-deficient F9 cells with DNA from a tripartite plasmid genome consisting of the pBR322 genome, the SV40 genome, and the HSV-1 tk gene. HSV-1 tk is present in both stem and differentiated cells; SV40 early proteins are present in differentiated cells but not in stem cells; the pBR322 genome is not expressed in either cell type. The SV40 and pBR322 genomes are more sensitive to DNase I digestion in stem cells than in differentiated cells, reflecting the DNase I- hypersensitivity of total stem-cell chromatin. The HSV-1 tk gene is the least sensitive to DNase I digestion in both cell types.
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Authors | K Huebner, A Linnenbach, S Weidner, G Glenn, C M Croce |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 78
Issue 8
Pg. 5071-5
(Aug 1981)
ISSN: 0027-8424 [Print] United States |
PMID | 6272288
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Chromatin
- DNA, Bacterial
- Thymidine Kinase
- Deoxyribonucleases
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Topics |
- Animals
- Cell Differentiation
- Cells, Cultured
- Chromatin
(ultrastructure)
- DNA, Bacterial
(metabolism)
- Deoxyribonucleases
(metabolism)
- Gene Expression Regulation
- Genes, Viral
- Mice
- Plasmids
- Simian virus 40
(genetics)
- Simplexvirus
(enzymology)
- Teratoma
(physiopathology)
- Thymidine Kinase
(genetics)
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