Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.

Abstract

Novikoff ascites tumor cell homogenate was found to catalyze the transfer of [14C]N-acetylglucosamine from UDP-[14C]GlcNAc to asialo-alpha 1-acid glycoprotein. Mucins appeared to be poor acceptors. Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl residues were introduced to position C-3 and C-6 of the terminal galactoses on the glycoprotein. It is concluded that Novikoff cells contain two N-acetylglucosaminyltransferases which might be involved in the synthesis of linear and branched forms of cell surface polylactosaminoglycans and blood group I/i antigenic structures.

• Authors: D H van den Eijnden, H Winterwerp, P Smeeman, W E Schiphorst
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 258 Issue 6 Pg. 3435-7 (Mar 25 1983) ISSN: 0021-9258 [Print] United States
• PMID: 6219989 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cations, Divalent
• N-Acetylglucosaminyltransferases
• N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
• N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase

Topics

• Animals
• Cations, Divalent
• Kinetics
• Liver Neoplasms, Experimental (enzymology)
• N-Acetylglucosaminyltransferases (metabolism)
• Rats
• Rats, Inbred Strains
• Substrate Specificity