Human monoclonal antibody against ganglioside GD2: use in development of enzyme-linked immunosorbent assay for the monitoring of anti-GD2 in cancer patients.

Abstract

Ganglioside GD2 is expressed on membranes of human melanoma cells and induces antibody responses in patients with melanoma. In this study a new enzyme-linked immunosorbent assay was developed for detection of human antibody against GD2. A human IgM monoclonal anti-GD2 antibody was used for development of the assay. The antigen, GD2, was prepared from human brain. Four micrograms of GD2 was required to coat a well in a polystyrene microtiter plate. As little as 10 ng of antibody could be detected. The applicability of the assay for detection of serum anti-GD2 antibody was demonstrated with sera from patients immunized with GD2-positive melanoma cell vaccine.

• Authors: T Tai, L D Cahan, J C Paulson, R E Saxton, R F Irie
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 73 Issue 3 Pg. 627-33 (Sep 1984) ISSN: 0027-8874 [Print] United States
• PMID: 6206271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigen-Antibody Complex
• Epitopes
• Gangliosides
• ganglioside, GD2

Topics

• Antibodies, Monoclonal
• Antigen-Antibody Complex
• Carbohydrate Sequence
• Cell Line
• Enzyme-Linked Immunosorbent Assay
• Epitopes (analysis)
• Gangliosides (analysis, immunology)
• Humans
• Leukemia, Lymphoid
• Neoplasms (immunology)
• Structure-Activity Relationship