Abstract |
The flame retardant tris(2,3-dibromopropyl)phosphate (Tris-BP) and the hair-dye component 2,4-diaminoanisole (2,4-DAA) were studied by possible cytotoxic effects in rat hepatoma cells grown in culture and in suspensions of isolated rat hepatocytes. Cell growth of Reuber cells was inhibited by 50% at 50 microgram/ml Tris-BP and 20 microgram/ml 2,4-DAA, respectively. At 200 microgram/ml Tris-BP protein synthesis in Reuber cells was reduced by 40%, whereas 50% inhibition of protein synthesis in isolated hepatocytes was seen at 100 microgram/ml. IC50 of 2,4-DAA with respect to protein synthesis was found at 400 microgram/ml in Reuber cells and at 3600 microgram/ml in MH1C1 cells, whereas in the isolated hepatocytes IC50 was 650 microgram/ml. DNA synthesis was inhibited by 50% at 225 microgram/ml Tris-BP in Reuber cells. At 500 microgram/ml 2,4-DAA DNA synthesis in Reuber and MH1C1 cells was inhibited by more than 80%.
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Authors | E J Søderlund, E Dybing |
Journal | Acta pharmacologica et toxicologica
(Acta Pharmacol Toxicol (Copenh))
Vol. 44
Issue 4
Pg. 284-8
(Apr 1979)
ISSN: 0001-6683 [Print] Denmark |
PMID | 433622
(Publication Type: Journal Article)
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Chemical References |
- Anisoles
- Flame Retardants
- Hair Dyes
- Mutagens
- Phosphates
- DNA
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Topics |
- Animals
- Anisoles
(toxicity)
- Cell Division
(drug effects)
- Cells, Cultured
- DNA
(biosynthesis)
- Flame Retardants
(toxicity)
- Hair Dyes
(toxicity)
- Liver
(cytology, drug effects)
- Liver Neoplasms, Experimental
(pathology)
- Male
- Mutagens
- Phosphates
(toxicity)
- Protein Biosynthesis
- Rats
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