A self-assembled nanoparticle vaccine based on pseudorabies virus glycoprotein D induces potent protective immunity against pseudorabies virus infection.

Abstract

Pseudorabies virus (PRV) mainly causes pseudorabies (PR) or Aujeszky's disease in pigs and can infect humans, raising public health concerns about zoonotic and interspecies transmission of PR. With the emergence of PRV variants in 2011, the classic attenuated PRV vaccine strains have failed to protect many swine herds against PR. Herein, we developed a self-assembled nanoparticle vaccine that induces potent protective immunity against PRV infection. PRV glycoprotein D (gD) was expressed using the baculovirus expression system and further presented on the lumazine synthase (LS) 60-meric protein scaffolds via the SpyTag003/SpyCatcher003 covalent coupling system. In mouse and piglet models, LSgD nanoparticles emulsified with the ISA 201VG adjuvant elicited robust humoral and cellular immune responses. Furthermore, LSgD nanoparticles provided effective protection against PRV infection and eliminated pathological symptoms in the brain and lungs. Collectively, the gD-based nanoparticle vaccine design appears to be a promising candidate for potent protection against PRV infection.

• Authors: Xujiao Ren, Nan Cao, Linxing Tian, Wenqiang Liu, Hechao Zhu, Zhenxiang Rong, Manman Yao, Xiangmin Li, Ping Qian
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 284 Pg. 109799 (Sep 2023) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 37327558 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Elsevier B.V. All rights reserved.

Chemical References

• pseudorabies virus glycoproteins
• Adjuvants, Immunologic
• Vaccines, Attenuated
• Pseudorabies Vaccines

Topics

• Humans
• Animals
• Swine
• Mice
• Herpesvirus 1, Suid
• Pseudorabies
• Adjuvants, Immunologic
• Vaccines, Attenuated
• Swine Diseases
• Pseudorabies Vaccines
• Rodent Diseases