Abstract |
Intravenous immunoglobulin ( IVIG) is a plasma-derived polyclonal IgG used for treatment of autoimmune diseases. Studies show that α-2,6 sialylation of the Fc improves anti-inflammatory activity. Also, afucosylation of the Fc efficiently blocks FcγRIIIA by increasing monovalent affinity to this receptor, which can be beneficial for treatment of refractory immune thrombocytopenia ( ITP). Here, we generated genome-edited chickens that synthesize human IgG1 Fc in the liver and secrete α-2,6 sialylated and low-fucosylated human IgG1 Fc (rhIgG1 Fc) into serum and egg yolk. Also, rhIgG1 Fc has higher affinity for FcγRIIIA than commercial IVIG. Thus, rhIgG1 Fc efficiently inhibits immune complex-mediated FcγRIIIA crosslinking and subsequent ADCC response. Furthermore, rhIgG1 Fc exerts anti-inflammatory activity in a passive ITP model, demonstrating chicken liver derived rhIgG1 Fc successfully recapitulated efficacy of IVIG. These results show that genome-edited chickens can be used as a production platform for rhIgG1 Fc with beneficial N-glycosylation pattern for anti-inflammatory activities.
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Authors | Jin Se Park, Hee Jung Choi, Kyung Min Jung, Kyung Youn Lee, Ji Hyeon Shim, Kyung Je Park, Young Min Kim, Jae Yong Han |
Journal | Communications biology
(Commun Biol)
Vol. 6
Issue 1
Pg. 589
(06 01 2023)
ISSN: 2399-3642 [Electronic] England |
PMID | 37264071
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2023. The Author(s). |
Chemical References |
- Immunoglobulin G
- Immunoglobulins, Intravenous
- Anti-Inflammatory Agents
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Topics |
- Humans
- Animals
- Immunoglobulin G
- Immunoglobulins, Intravenous
(pharmacology)
- Chickens
(metabolism)
- Glycosylation
- Anti-Inflammatory Agents
(pharmacology)
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