Abstract | OBJECTIVE: METHODS: The expression of EpCAM in ovarian cancer tissues was analyzed by databases. The EpCAM expression and immune cell infiltration in different foci of ovarian cancer were detected by 8-channel flow cytometry. The toxic effect of M701 on OVCAR3 was tested using the in vitro cytotoxicity assay. The 3D cell culture and drug intervention experiments were performed to evaluate the therapeutic effect of M701 in ovarian cancer specimens. Flow cytometry was used to examine the effect of M701 on the binding of immune cells to tumor cells and the activation capacity of T cells. RESULTS: The results of the bioinformatic analysis showed that the expression of EpCAM in ovarian cancer tissue was significantly higher than that in normal ovarian tissue. The 8-channel flow cytometry of clinical samples showed that the EpCAM expression and lymphocyte infiltration were significantly heterogeneous among ovarian cancer patients and lesions at different sites. The in vitro experiment results showed that M701 had a significant killing effect on OVCAR3 cells. M701 also obviously killed primary tumor cells derived from some patients with ovarian cancer ascites. M701 could mediate the binding of CD3+ T cells to EpCAM+ tumor cells and induce T cell activation in a dose-dependent manner. CONCLUSION:
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Authors | Yi-Nuo Li, Yuan-Yuan Li, Shi-Xuan Wang, Xiang-Yi Ma |
Journal | Current medical science
(Curr Med Sci)
Vol. 43
Issue 3
Pg. 539-550
(Jun 2023)
ISSN: 2523-899X [Electronic] China |
PMID | 37119369
(Publication Type: Journal Article)
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Copyright | © 2023. Huazhong University of Science and Technology. |
Chemical References |
- Epithelial Cell Adhesion Molecule
- Cell Adhesion Molecules
- Antigens, Neoplasm
- Antibodies, Bispecific
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Topics |
- Female
- Humans
- Epithelial Cell Adhesion Molecule
(genetics, therapeutic use)
- Ovarian Neoplasms
(drug therapy)
- Ascites
- Cell Adhesion Molecules
(genetics)
- Antigens, Neoplasm
- Apoptosis
- Cell Line, Tumor
- Antibodies, Bispecific
(pharmacology)
- Immunotherapy
(methods)
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