Interleukin-1β (IL-1β) is one of the most potent pro-inflammatory
cytokines implicated in a wide range of autoinflammatory, autoimmune, infectious, and degenerative diseases. Therefore, many researchers have focused on developing therapeutic molecules that inhibit IL-1β-IL-1 receptor 1 (IL-1R1) interaction for the treatment of IL-1-related diseases. Among IL-1-related diseases,
osteoarthritis (OA), is characterized by progressive cartilage destruction, chondrocyte
inflammation, and extracellular matrix (ECM) degradation.
Tannic acid (TA) has been proposed to have multiple beneficial effects, including anti-inflammatory,
anti-oxidant, and anti-
tumor activities. However, it is unclear whether TA plays a role in anti-IL-1β activity by blocking IL-1β-IL-1R1 interaction in OA. In this study, we report the anti-IL-1β activity of TA in the progression of OA in both in vitro human OA chondrocytes and in vivo rat OA models. Herein, using-ELISA-based screening, natural compound candidates capable of inhibiting the IL-1β-IL-1R1 interaction were identified. Among selected candidates, TA showed hindering IL-1β-IL-1R1 interaction by direct binding to IL-1β using surface plasmon resonance (SPR) assay. In addition, TA inhibited IL-1β bioactivity in HEK-Blue IL-1-dependent reporter cell line. TA also inhibited IL-1β-induced expression of
inducible nitric oxide synthase (NOS2),
cyclooxygenase-2 (COX-2),
IL-6,
tumor necrosis factor-alpha (TNF-α),
nitric oxide (NO), and
prostaglandin E2 (
PGE2) in human OA chondrocytes. Moreover, TA downregulated IL-1β-stimulated
matrix metalloproteinase (
MMP)3, MMP13, ADAM
metallopeptidase with
thrombospondin type 1 motif (ADAMTS)4, and ADAMTS5, while upregulating
collagen type II (COL2A1) and
aggrecan (ACAN). Mechanistically, we confirmed that TA suppressed IL-1β-induced MAPK and NF-κB activation. The protective effects of TA were also observed in a monosodium
iodoacetamide (MIA)-induced rat OA model by reducing
pain and cartilage degradation and inhibiting IL-1β-mediated
inflammation. Collectively, our results provide evidence that TA plays a potential role in OA and IL-1β-related diseases by hindering IL-1β-IL-1R1 interaction and suppressing IL-1β bioactivity.