Abstract |
A monosaccharide precursor of Escherichia coli lipid A, designated lipid X, which is a diacylglucosamine 1-phosphate with beta-hydroxymyristoyl groups at positions 2 and 3, was shown to have the ability to induce the production of tumor necrosis factor (TNF)-like tumor-cytotoxic factor by a murine macrophage-like cell line, J774.1. This cytotoxic factor was released from J774.1 cells grown in the presence of lipid X and related compounds, and it was assayed as to its lytic activity against [3H] thymidine-labeled L929 cells. Dose-response studies revealed that lipid X induced the production of smaller amounts of the tumor-cytotoxic factor than LPS at low concentrations, but it induced that of considerable amounts at and over 1 microgram/ml. Elimination of 1-phosphate or 3-O-beta-hydroxymyristoyl group from lipid X completely prevented the induction of producing this factor by the macrophages. Therefore, it is suggested that both 1-phosphate and 3-O-beta-hydroxymyristoyl groups are essential for the biologic activity of lipid X, as to the induction of the tumor-cytotoxic factor production in the macrophages.
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Authors | F Amano, M Nishijima, Y Akamatsu |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 136
Issue 11
Pg. 4122-7
(Jun 01 1986)
ISSN: 0022-1767 [Print] United States |
PMID | 3701065
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glycolipids
- Glycoproteins
- Lipid A
- Lipopolysaccharides
- Monosaccharides
- Tumor Necrosis Factor-alpha
- lipid X
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Topics |
- Animals
- Cell Line
- Cytotoxicity, Immunologic
- Glycolipids
(immunology, pharmacology)
- Glycoproteins
(biosynthesis)
- Lipid A
(pharmacology)
- Lipopolysaccharides
(pharmacology)
- Macrophage Activation
- Macrophages
(immunology, metabolism)
- Mice
- Monosaccharides
(pharmacology)
- Neoplasms, Experimental
(immunology, pathology)
- Tumor Necrosis Factor-alpha
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