As a result of bright complexation properties, easy functionalization and the ability to self-organize in an aqueous
solution, amphiphilic supramolecular macrocycles are being actively studied for their application in nanomedicine (drug delivery systems, therapeutic and
theranostic agents, and others). In this regard, it is important to study their potential toxic effects. Here, the synthesis of amphiphilic calix[4]
resorcinarene carboxybetaines and their
esters and the study of a number of their microbiological properties are presented: cytotoxic effect on normal and
tumor cells and effect on cellular and non-cellular components of blood (hemotoxicity, anti-platelet effect, and
anticoagulant activity). Additionally, the interaction of macrocycles with
bovine serum albumin as a model
plasma protein is estimated by various methods (fluorescence spectroscopy, synchronous fluorescence spectroscopy, circular dichroic spectroscopy, and dynamic light scattering). The results demonstrate the low toxicity of the macrocycles, their anti-platelet effects at the level of
acetylsalicylic acid, and weak
anticoagulant activity. The study of BSA-macrocycle interactions demonstrates the dependence on macrocycle hydrophilic/hydrophobic group structure; in the case of carboxybetaines, the formation of complexes prevents self-aggregation of BSA molecules in
solution. The present study demonstrates new data on potential
drug delivery nanosystems based on amphiphilic calix[4]resorcinarenes for their cytotoxicity and effects on blood components.