Abstract | OBJECTIVES: METHODS: An integrative analysis combining data derived from the Gene Expression Omnibus (GEO) and cBioPortal databases was performed to investigate the potential molecular biomarker. Immunohistochemistry and quantitative real-time polymerase chain reactions were used to measure the expression of messenger ribonucleic acid ( mRNA) and protein by OLFM4. In combination with the gastroscopic findings and the OLFM4 expression in GIM-GC, a predictive model was established. The receiver operator characteristic curve (ROC) was applied to assess the diagnostic value of the model for GIM-GC. RESULTS: According to the GEO and cBioPortal databases, OLFM4 was identified as a key gene in the diagnosis of GIM-GC. Higher protein expression of OLFM4 was found in GIM and GIM-GC compared with chronic superficial gastritis (GS) (p < 0.05). The positive expression rate of OLFM4 in paracancerous tissue (GCP) was higher than in GIM (p > 0.05). There was no significant difference between GIM-GC and GCP (p > 0.05). The mRNA expression of OLFM4 was similar to the protein expression, and the positive expression rate was higher in early GIM-GC than in GIM (p < 0.05). CONCLUSION:
Olfactomedin 4 could be used as a biomarker for the early diagnosis of GIM-GC, and the logistic predictive model could be an effective tool for increasing the early diagnostic rate.
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Authors | Lixing Pang, Xin Yan, Dongxing Su, Xianbin Wu, Haixing Jiang |
Journal | Scandinavian journal of gastroenterology
(Scand J Gastroenterol)
Vol. 58
Issue 2
Pg. 133-141
(Feb 2023)
ISSN: 1502-7708 [Electronic] England |
PMID | 36124708
(Publication Type: Journal Article)
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Chemical References |
- olfactomedin
- Biomarkers
- RNA, Messenger
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Topics |
- Humans
- Stomach Neoplasms
(diagnosis, genetics)
- Feasibility Studies
- Early Detection of Cancer
- Biomarkers
- Metaplasia
(genetics)
- RNA, Messenger
- Precancerous Conditions
(diagnosis, genetics)
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