PHGDH attaches importance to
serine biosynthesis in
cancer cells and maintaining mitochondrial redox homeostasis. However, the role of PHGDH inhibitor
CBR-5884 in cell ROS level and its downstream pathways has not been explored in
epithelial ovarian cancer. Thus, we investigated the function and possible mechanism of PHGDH inhibitor
CBR-5884 on
epithelial ovarian cancer in vitro and in vivo. A2780, OVCAR3, and ES-2 were treated with
CBR-5884 at different concentrations or different time points. Results showed that
CBR-5884 inhibited
epithelial ovarian cancer cell proliferation, migration, and invasion and increases cell ROS level. Meanwhile,
CBR-5884 exerts antitumor effect through activating ROS/Wnt/β-
catenin pathway. Besides,
CBR-5884 exerts antitumor effect in vivo. What's more, we explored the effect of
CBR-5884 with or without
PARP inhibitor Olaparib, which showed that the two together had a larger effect. In conclusion, PHGDH inhibitor
CBR-5884 inhibits
epithelial ovarian cancer proliferation, migration, and invasion through activating ROS/Wnt/β-
catenin pathway and plays a synergistic role with
PARP inhibitor olaparib, which provided a theoretical basis for PHGDH inhibitor
CBR-5884 in clinical treatment.