Phomoxanthone A Targets ATP Synthase.

Abstract

Phomoxanthone A is a naturally occurring molecule and a powerful anti-cancer agent, although its mechanism of action is unknown. To facilitate the determination of its biological target(s), we used affinity-based labelling using a phomoxanthone A probe. Labelled proteins were pulled down, subjected to chemoproteomics analysis using LC-MS/MS and ATP synthase was identified as a likely target. Mitochondrial ATP synthase was validated in cultured cells lysates and in live intact cells. Our studies show sixty percent inhibition of ATP synthase by 260 μM phomoxanthone A.

• Authors: Rameez Ali, Sangram S Parelkar, Paul R Thompson, Susan Mitroka-Batsford, Siddartha Yerramilli, Suzanne F Scarlata, Katelyn S Mistretta, Jeannine M Coburn, Anita E Mattson
• Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (Chemistry) Vol. 28 Issue 70 Pg. e202202397 (Dec 15 2022) ISSN: 1521-3765 [Electronic] Germany
• PMID: 36082977 (Publication Type: Journal Article)
• Copyright: © 2022 Wiley-VCH GmbH.

Chemical References

• phomoxanthone A
• Mitochondrial Proton-Translocating ATPases
• Affinity Labels
• Adenosine Triphosphate

Topics

• Chromatography, Liquid
• Tandem Mass Spectrometry
• Mitochondrial Proton-Translocating ATPases (metabolism)
• Affinity Labels
• Adenosine Triphosphate (metabolism)