Abstract |
Signaling by the human C-type lectin-like receptor, natural killer (NK) cell inhibitory receptor NKR-P1, has a critical role in many immune-related diseases and cancer. C-type lectin-like receptors have weak affinities to their ligands; therefore, setting up a comprehensive model of NKR-P1-LLT1 interactions that considers the natural state of the receptor on the cell surface is necessary to understand its functions. Here we report the crystal structures of the NKR-P1 and NKR-P1:LLT1 complexes, which provides evidence that NKR-P1 forms homodimers in an unexpected arrangement to enable LLT1 binding in two modes, bridging two LLT1 molecules. These interaction clusters are suggestive of an inhibitory immune synapse. By observing the formation of these clusters in solution using SEC-SAXS analysis, by dSTORM super-resolution microscopy on the cell surface, and by following their role in receptor signaling with freshly isolated NK cells, we show that only the ligation of both LLT1 binding interfaces leads to effective NKR-P1 inhibitory signaling. In summary, our findings collectively support a model of NKR-P1:LLT1 clustering, which allows the interacting proteins to overcome weak ligand-receptor affinity and to trigger signal transduction upon cellular contact in the immune synapse.
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Authors | Jan Bláha, Tereza Skálová, Barbora Kalousková, Ondřej Skořepa, Denis Cmunt, Valéria Grobárová, Samuel Pazicky, Edita Poláchová, Celeste Abreu, Jan Stránský, Tomáš Kovaľ, Jarmila Dušková, Yuguang Zhao, Karl Harlos, Jindřich Hašek, Jan Dohnálek, Ondřej Vaněk |
Journal | Nature communications
(Nat Commun)
Vol. 13
Issue 1
Pg. 5022
(08 26 2022)
ISSN: 2041-1723 [Electronic] England |
PMID | 36028489
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Antigens, Surface
- Lectins, C-Type
- Ligands
- NK Cell Lectin-Like Receptor Subfamily B
- Receptors, Cell Surface
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Topics |
- Antigens, Surface
- Cluster Analysis
- Humans
- Killer Cells, Natural
- Lectins, C-Type
- Ligands
- NK Cell Lectin-Like Receptor Subfamily B
- Receptors, Cell Surface
- Scattering, Small Angle
- Synapses
- X-Ray Diffraction
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