Chicken Muscle Protein-Derived Peptide VVHPKESF Reduces TNFα-Induced Inflammation and Oxidative Stress by Suppressing TNFR1 Signaling in Human Vascular Endothelial Cells.

Abstract

SCOPE:

This study aims to investigate the protective effects of four chicken muscle-derived peptides Val-Arg-Pro (VRP), Leu-Lys-Tyr (LKY), Val-Arg-Tyr (VRY), and Val-Val-His-Pro-Lys-Glu-Ser-Phe [VVHPKESF (V-F)] on tumor necrosis factor alpha (TNFα)-induced endothelial inflammation and oxidative stress in human vascular endothelial EA.hy926 cells.

METHODS AND RESULTS:

Inflammation and oxidative stress are induced in EA.hy926 cells by TNFα (10 ng mL-1 ) treatment for different periods of time. Inflammatory proteins and signaling molecules including inducible nitric oxide synthase, intracellular cell adhesion molecule-1, vascular cell adhesion molecule-1 (VCAM-1), cyclooxygenase 2 (COX2), nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPKs), and TNFα receptor 1 (TNFR1) are measured by qRT-PCR or western blotting; soluble TNFR1 level and nicotinamide adenine dinucleotide phosphate NADPH) oxidase activity are determined by Elisa kits; superoxide is measured by dihydroethidium staining. Only V-F treatment inhibits the expression of VCAM-1 and COX2, via suppressing NF-κB and p38 MAPK signaling, respectively, while reduced oxidative stress via the inhibition of NADPH oxidase activity; V-F treatment attenuates both gene and protein expressions of TNFR1.

CONCLUSION:

V-F treatment ameliorates TNFα-induced endothelial inflammation and oxidative stress likely via the inhibition of TNFR1 signaling, suggesting its potential as a functional food ingredient or nutraceutical in the prevention and treatment of hypertension and cardiovascular diseases.

• Authors: Hongbing Fan, Khushwant S Bhullar, Zihan Wang, Jianping Wu
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 66 Issue 17 Pg. e2200184 (09 2022) ISSN: 1613-4133 [Electronic] Germany
• PMID: 35770889 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 Wiley-VCH GmbH.

Chemical References

• Muscle Proteins
• NF-kappa B
• Receptors, Tumor Necrosis Factor, Type I
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• Cyclooxygenase 2

Topics

• Animals
• Chickens (metabolism)
• Cyclooxygenase 2 (genetics, metabolism)
• Endothelial Cells
• Humans
• Inflammation (metabolism)
• Muscle Proteins (metabolism)
• NF-kappa B (metabolism)
• Oxidative Stress
• Receptors, Tumor Necrosis Factor, Type I (metabolism)
• Tumor Necrosis Factor-alpha (metabolism, pharmacology)
• Vascular Cell Adhesion Molecule-1 (genetics, metabolism)