Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins.

Abstract

Due to the lower risks of adverse effects, nonviral gene therapy is a suitable alternative to transfect cancer cells with a suicide gene to let them kill themselves by expressing toxic ribosome-inactivating proteins. Plasmids are stable and easy-to-produce vectors, but they have some disadvantages due to the bacterial backbone. Applying the minicircle technology, this problem can be solved with manageable effort in a well-equipped laboratory. With the described methodology, minicircle-DNA can be produced at low costs. The cell killing properties are monitored following transfection using the CytoSMART® Omni system-a camera based live cell imaging device.

• Authors: Alexander Sonntag, Hardy Mitdank, Alexander Weng
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2521 Pg. 157-171 ( 2022) ISSN: 1940-6029 [Electronic] United States
• PMID: 35732997 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• Ribosome Inactivating Proteins

Topics

• Genetic Vectors (genetics)
• Humans
• Plasmids (genetics)
• Ribosome Inactivating Proteins
• Ribosomes (genetics)
• Transfection