Abstract |
Anthrax protective antigen (PA), the receptor-binding component of anthrax toxin, elicits toxin- neutralizing antibodies which provide protection against anthrax disease. PA binds to two mammalian receptors, capillary morphogenesis protein-2 (CMG2) and tumor endothelial marker-8 (TEM8). We previously observed that binding of PA to its receptors plays a role in eliciting a strong toxin- neutralizing antibody response. In this study, we examined the roles that individual receptors play in mediating the toxin- neutralizing antibody response. Mice immunized with PA that binds preferentially to CMG2 elicited a toxin- neutralizing antibody response similar to that elicited by wild-type PA, whereas the antibody response elicited by PA that binds preferentially to TEM8 was significantly lower. Also, the toxin- neutralizing antibody response elicited by wild-type PA in CMG2-null mice was found to be significantly lower than that induced in CMG2-sufficient mice, further supporting a predominant role for the CMG2 receptor in mediating a protective antibody response to PA.
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Authors | Anita Verma, Mahtab Moayeri, Christopher P Mocca, Danielle O'Mard, Qian Ma, Stephen H Leppla, Drusilla L Burns |
Journal | Vaccine
(Vaccine)
Vol. 40
Issue 32
Pg. 4318-4321
(07 30 2022)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 35710508
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Intramural)
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Copyright | Published by Elsevier Ltd. |
Chemical References |
- Antibodies, Neutralizing
- Antigens, Bacterial
- Bacterial Toxins
- Receptors, Peptide
- anthrax toxin
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Topics |
- Animals
- Anthrax
- Antibodies, Neutralizing
- Antigens, Bacterial
- Bacterial Toxins
- Mammals
(metabolism)
- Mice
- Morphogenesis
- Receptors, Peptide
(genetics, metabolism)
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