The feasibility of using an autologous GM-CSF-secreting breast cancer vaccine to induce immunity in patients with stage II-III and metastatic breast cancers.

Abstract

PURPOSE:

The antigenic targets of immunity and the role of vaccination in breast cancer are unknown. We performed a phase I study of an autologous GM-CSF-secreting breast cancer vaccine in patients with metastatic and stage II-III breast cancer.

METHODS:

Tumor cells from patients with metastatic (n = 15) and stage II-III (n = 7) disease were transduced with a replication-defective adenoviral vector encoding GM-CSF, and then irradiated. Twelve and seven patients with metastatic and stage II-III disease, respectively, received weekly vaccination for three weeks, followed by every other week until disease progression or vaccine supply was exhausted (metastatic) or until six total vaccine doses were administered (stage II-III).

RESULTS:

Among those patients with metastatic disease who received vaccinations, eight had progressive disease at two months, three had stable disease for 4-13 months, and one has had no evidence of disease for 13 years. Of the patients with stage II-III disease, five died of metastatic disease between 1.16 and 8.49 years after the start of vaccinations (median 6.24 years) and two are alive as of September 2021. Toxicities included injection site reactions, fatigue, fever, upper respiratory symptoms, joint pain, nausea, and edema. Four of five evaluable patients with metastatic disease developed a skin reaction with immune cell infiltration after the fifth injection of unmodified, irradiated tumor cells.

CONCLUSION:

We conclude that tumor cells can be harvested from patients with metastatic or stage II-III breast cancer to prepare autologous GM-CSF-secreting vaccines that induce coordinated immune responses with limited toxicity. TRIAL REGISTRATION AND DATE OF REGISTRATION: clinicaltrials.gov, NCT00317603 (April 25, 2006) and NCT00880464 (April 13, 2009).

• Authors: Karen S Anderson, Timothy K Erick, Meixuan Chen, Heather Daley, Margaret Campbell, Yolonda Colson, Martin Mihm, Labib R Zakka, Marika Hopper, William Barry, Eric P Winer, Glenn Dranoff, Beth Overmoyer
• Journal: Breast cancer research and treatment (Breast Cancer Res Treat) Vol. 194 Issue 1 Pg. 65-78 (Jul 2022) ISSN: 1573-7217 [Electronic] Netherlands
• PMID: 35482127 (Publication Type: Clinical Trial, Phase I, Journal Article)
• Copyright: © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• Cancer Vaccines
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Breast Neoplasms (immunology, pathology, therapy)
• Cancer Vaccines (toxicity)
• Feasibility Studies
• Female
• Genetic Vectors
• Granulocyte-Macrophage Colony-Stimulating Factor (genetics)
• Humans