Characterization of prodigiosin pigment by Serratia marcescens and the evaluation of its bioactivities.

Abstract

The aim of the present study is to discover a bacterial pigment providing protection and prevention of neurological damage and cancer development, which can have a role as a non-synthetic food additive in the food industry as well as an active drug ingredient of anticancer drugs and pharmaceuticals for neural injury. Within this scope, Serratia marcescens MB703 strain was used to produce prodigiosin. Characterization of the prodigiosin was carried out using UV-VIS, and FT-IR. In addition, its inhibitory action on AChE and antioxidant activities were determined. The cytotoxic, genotoxic and antigenotoxic activities of the prodigiosin as well as its antiproliferative activities were detected. It was determined that the maximum production of the prodigiosin (72 mg/L). The prodigiosin was found to cause no significant difference in its inhibitory effect on AChE. The prodigiosin was found effective on all antioxidant parameters tested. The IC50 values of the prodigiosin on SK-MEL-30 and HT-29 cells were calculated as 70 and 47 μM, respectively. This IC50 values of the prodigiosin showed no cytotoxic effect on L929 cells. Prodigiosin did not have genotoxic effect alone and also seem to decrease DNA damage induced by H2O2 in L929 cells. The findings of in vitro experimental studies suggest that using the prodigiosin pigment as a drug candidate for cancer and neurodegenerative disease therapy is both effective and safe.

• Authors: Merve Tunca Koyun, Seda Sirin, Belma Aslim, Gokce Taner, Serap Nigdelioglu Dolanbay
• Journal: Toxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 82 Pg. 105368 (Aug 2022) ISSN: 1879-3177 [Electronic] England
• PMID: 35476923 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• Antioxidants
• Hydrogen Peroxide
• Prodigiosin

Topics

• Antineoplastic Agents (pharmacology)
• Antioxidants (pharmacology)
• Humans
• Hydrogen Peroxide
• Neurodegenerative Diseases
• Prodigiosin (pharmacology)
• Serratia marcescens (genetics)
• Spectroscopy, Fourier Transform Infrared