Accurate
drug delivery is a common topic, and it has always been an aim that scientists strive to achieve. To address this need, multifunctional and stimulus-sensitive nanoplatforms have attracted significant attention. Here we fabricated a
glutathione (GSH) and adenosine-5'-triphosphate (
ATP) dual-sensitive nanoplatform for controlled drug release and activatable MRI of
tumors based on
DNA aptamer and
manganese dioxide (MnO2) nanosheets. Cleverly utilizing the
DNA tunability,
AS1411 aptamer which binds
nucleolin, a
protein specifically expressed on
tumor-associated endothelial cells, was designed with
ATP aptamer and its
cDNA to load the anticancer
drug,
doxorubicin (Dox). The formed
DNA-Dox complex was delivered to the
tumor region with the help of MnO2 nanosheets and
AS1411 aptamer. Then, the on-demand drug release in
tumor cells was realized with the co-effect of the
ATP aptamer and GSH reduction. It was found that without the structure of the MnO2 nanosheets being broken by GSH, Dox almost could not be released even in the presence of
ATP. Similarly, without
ATP, Dox was still maintained in the duplex even with GSH. Further combining the MRI ability and chemodynamic
therapy of the produced Mn2+, an improved effect of the inhibition of
tumor growth and imaging was achieved. Our designed
DNA aptamer-based dual-responsive nanoplatform can realize the targeted
drug delivery and MRI of
breast tumor cells both in vitro and in vivo.