Background: Using a variety of chemical compounds and biomolecules, researchers have been working on new
antidiabetic drugs for many years. Anti-diabetic research is increasingly using nanomaterials because of their unique qualities, such as their tiny size, biocompatibility, and ability to penetrate cell membranes for
drug delivery. Using extract of T. couneifolia coated with
silver nanoparticles as a model for
diabetes mellitus research was one of the goals of this work. Methods: Uv-Vis spectroscopy was used to measure the TAgNPs surface plasmon resonance. FTIR spectroscopy confirmed the attached functional groups, XRD analysis confirmed the size and crystallinity, scanning electron microscopy revealed that the majority of the particles were spherical, and EDX performed the elemental analysis. For 21 days,
alloxan-induced diabetic Wistar rats (N = 25, n = 5/group) were administered 10 mg/kg
body weight of photosynthesized AgNPs as a standard animal model, while those in the untreated normal control group C, received distilled water as a control, diabetics who were treated with 0.5 mg/kg of
body weight of
glibenclamide, 10 mg/kg of methanolic T. couneifolia extract, and diabetics who were given 10 mg/kg of
body weight of synthetic AgNPs derived from T. couneifolia in the DAgNPs group. At the conclusion of the treatment,
lipid, liver and kidney profiles were re-examined to determine whether or not the treatment had been effective (day 21). Oral
glucose doses of 2 g/kg of
body weight were administered to each group, and
blood glucose levels were measured at various intervals (day 21). Fasting
glucose levels were measured using a glucometer. Each animal's urine was tested for leukocytes,
nitrites, and
bilirubin using lab-made prepared assay kits. One-way ANOVA and Dunnett's test were used for statistical analysis. Results: The surface plasmon resonance effect was examined with UV-vis, it showed a sharp peak at 412 nm. X-ray diffraction measurements indicated that the produced nanoparticles were between 15 to 31.44 nm in size.
Alloxan-induced diabetic rats were fed AgNPs derived from phytosynthesized AgNPs, compared to diabetic control rats, diabetic rats treated with AgNPs showed a considerable improvement in their
dyslipidemia status. Over the course of the days, it also lowered
blood glucose levels. A reduction in
blood glucose levels, a rise in
body weight, and significant improvements in the
lipid, liver, and renal profiles were also seen. Conclusions: The present findings revealed that plant mediated
silver nanoparticles significantly improved the
alloxan induced diabetic changes in various treated rats and might be used for the treatment of diabetes.