Fabrication and assessment of folic acid conjugated-chitosan modified PLGA nanoparticle for delivery of alpha terpineol in colon cancer.

Abstract

The objective of this study was to fabrication of α-terpineol-PLGA nanoparticles coated with folic acid-chitosan (αT-PCF-NPs) as well as evaluates their anticancer effects. αT-PCF-NPs were synthesized using the nanoprecipitation method and characterized by Dynamic light scattering (DLS), zeta potential (ZP), scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) analysis. Folic acid (FA) binding rate and entrapment efficiency of α-T were assessed by HPLC method. MTT assay was performed for cytotoxicity assessment. Quantitative polymerase chain reaction (qPCR) analysis, acridine orange and propodium iodide (AO/PI) staining and cell cycle analysis were done to assess the pro-apoptotic properties of αT-PCF-NPs. Molecular analysis for angiogenesis and antioxidant properties and murine colon cancer model for antitumor effects of αT-PCF-NPs were used. The % FA-binding and encapsulation efficiency of α-T in αT-PCF-NPs (particle size of 263.95 nm, polydispersity index (PDI) of 0.25, and surface charge of +38.20 mV) was reported to be 67% and 88.1% respectively. The higher inhibitory effect of αT-PCF-NPs on cancer cells compared to HFF cells was confirmed. The pro-apoptotic effect of αT-PCF-NPs was showed by increased SubG1 phase cells, AO/PI staining results and up and down regulation Bax and Bcl-2 as pro and anti-apoptotic genes in HT-29 cells. Antioxidant (SOD) and angiogenesis genes (VEGF and VEGF-R) were inhibited by αT-PCF-NPs exposure in HT-29 cells and also decreased the size of murine tumors was confirmed in exposure of αT-PCF-NPs. αT-PCF-NPs can be considered as a promising anticancer drug for colon cancer.

• Authors: Amir Rahmati, Masoud Homayouni Tabrizi, Ehsan Karimi, Bahar Zarei
• Journal: Journal of biomaterials science. Polymer edition (J Biomater Sci Polym Ed) Vol. 33 Issue 10 Pg. 1289-1307 (07 2022) ISSN: 1568-5624 [Electronic] England
• PMID: 35260045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Cyclohexane Monoterpenes
• Drug Carriers
• Vascular Endothelial Growth Factor A
• alpha-terpineol
• Chitosan
• Folic Acid

Topics

• Animals
• Antioxidants
• Chitosan (chemistry)
• Colonic Neoplasms (drug therapy)
• Cyclohexane Monoterpenes
• Drug Carriers (chemistry)
• Folic Acid
• Humans
• Mice
• Nanoparticles (chemistry)
• Particle Size
• Vascular Endothelial Growth Factor A