The role of splicing factor PRPF8 in breast cancer.

Abstract

BACKGROUND:

Alternative splicing is a mechanism to produce different proteins with diverse functions from one gene. Many splicing factors play an important role in cancer progression. PRPF8 is a core protein component of the spliceosome complex, U4/U6-U5 tri-snRNP.

OBJECTIVE:

However, PRPF8 involved in mRNA alternative splicing are rarely included in the prognosis.

METHODS:

We found that PRPF8 was expressed in all examined cancer types. Further analyses found that PRPF8 expression was significantly different between the breast cancer and paracancerous tissues.

RESULTS:

Survival analyses showed that PRPF8-high patients had a poor prognosis, and the expression of PRPF8 is associated with distant metastasis-free survival (DMFS) and post progression survival (PPS). Gene Set Enrichment Analysis (GSEA) has revealed that PRPF8 expression is correlated with TGF-β, JAK-STAT, and cell cycle control pathways. Consistent with these results, upon PRPF8 silencing, the growth of MCF-7 cells was reduced, the ability of cell clone formation was weakened, and p⁢21 expression was increased.

CONCLUSIONS:

These results have revealed that PRPF8 is a significant factor for splicing in breast cancer progression.

• Authors: Difei Cao, Jiaying Xue, Guoqing Huang, Jing An, Weiwei An
• Journal: Technology and health care : official journal of the European Society for Engineering and Medicine (Technol Health Care) Vol. 30 Issue S1 Pg. 293-301 ( 2022) ISSN: 1878-7401 [Electronic] Netherlands
• PMID: 35124606 (Publication Type: Journal Article)

Chemical References

• PRPF8 protein, human
• RNA Splicing Factors
• RNA-Binding Proteins
• Ribonucleoprotein, U4-U6 Small Nuclear
• Ribonucleoprotein, U5 Small Nuclear

Topics

• Breast Neoplasms (genetics)
• Female
• HeLa Cells
• Humans
• RNA Splicing Factors (genetics)
• RNA-Binding Proteins (genetics, metabolism)
• Ribonucleoprotein, U4-U6 Small Nuclear (genetics, metabolism)
• Ribonucleoprotein, U5 Small Nuclear (genetics, metabolism)