A label-free electrochemical immunosensor for CA125 detection based on CMK-3(Au/Fc@MgAl-LDH)n multilayer nanocomposites modification.

Abstract

A label-free electrochemical immunosensor was constructed for cancer antigen 125 (CA125) detection based on multiple-enlargement means of layer-by-layer (LBL) assembly of ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs) and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). A CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was designed using technology of LBL self-assembly among negatively charged Au NPs, positively charged CMK-3 and Fc@MgAl-LDH nanosheets. The CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was used as carriers to increase the immobilization of antibody and the number of loading Fc, conductors to strengthen conductivity and enhancers to amplify signal of Fc step-by-step. Besides, this special and excellent way of LBL assembly can immensely amplify the signal of immunosensor and more immobilize the biomolecules, and label-free method is a more simple the measuring way and the procedure. The immunosensor displayed a wider linear range of 0.01 U ml-1-1000 U ml-1 and a lower detection limit of 0.004 U ml-1. Therefore, the sensor can stablely and accurately be applied for CA125 detection in clinical cancer diagnosis.

• Authors: Mengdie Wu, Simin Liu, Feifan Qi, Ren Qiu, Jing Feng, Xinshui Ren, Shengzhong Rong, Hongkun Ma, Dong Chang, Hongzhi Pan
• Journal: Talanta (Talanta) Vol. 241 Pg. 123254 (May 01 2022) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 35101834 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• CA-125 Antigen
• Gold
• Graphite

Topics

• Biosensing Techniques (methods)
• CA-125 Antigen
• Electrochemical Techniques
• Gold (chemistry)
• Graphite (chemistry)
• Immunoassay (methods)
• Limit of Detection
• Metal Nanoparticles (chemistry)
• Nanocomposites (chemistry)