Rapid and efficient capturing of circulating tumor cells from breast cancer Patient's whole blood via the antibody functionalized microfluidic (AFM) chip.

Abstract

Accurate enumeration of circulating tumor cells (CTCs) in cancer patient's blood functions as a form of "liquid biopsy", which is pivotal for cancer screening, prognosis, and diagnosis. Herein, we demonstrate a novel antibody functionalized microfluidic (AFM) chip that rapidly and accurately qualifies CTCs from breast cancer patient's whole blood. The AFM chip consists of three buffering zones, and four main capturing zones filled with equilateral triangular pillars and periodically distributed obstacles. We validate the AFM chip with three Epithelial cell adhesion molecule (EpCAM) positive cancer cell lines, including breast (MCF-7), prostate (PC3), and lung cancer cell lines (A549), achieving capture efficiencies of 99.5%, 98.5%, and 96.72%, respectively, at a flow rate of 0.6 mL/hour. We further confirm the efficacy of the AFM chip with five advanced breast cancer patients' whole blood to capture EpCAM+/CK19+/CD45-/DAPI + CTCs. Interestingly, high number of CTCs were identified from each patient's 1 mL whole blood (595-2270), The AFM chip is highly efficient at rapidly capturing CTCs from cancer patients' whole blood without requiring extra equipment, which is critically beneficial for clinical application.

• Authors: Aynur Abdulla, Zhianan Zhang, Khan Zara Ahmad, Antony R Warden, Hengyu Li, Xianting Ding
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 201 Pg. 113965 (Apr 01 2022) ISSN: 1873-4235 [Electronic] England
• PMID: 35016111 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Epithelial Cell Adhesion Molecule

Topics

• Biosensing Techniques
• Breast Neoplasms
• Cell Line, Tumor
• Cell Separation
• Epithelial Cell Adhesion Molecule
• Humans
• Male
• Microfluidics
• Neoplastic Cells, Circulating