Proliferating
cancer cells have high energy demands, which is mainly obtained through glycolysis. The transmembrane trafficking of
lactate, a major metabolite produced by glycolytic
cancer cells, relies on monocarboxylate transporters (MCTs). MCT1 optimally imports
lactate, although it can work bidirectionally, and its activity has been linked to
cancer aggressiveness and poor outcomes.
AZD3965, a specific MCT1 inhibitor, was tested both in vitro and in vivo, with encouraging results; a phase I clinical trial has already been undertaken. Thus, analysis of the experimental evidence using
AZD3965 in different
cancer types could give valuable information for its clinical use. This systematic review aimed to assess the in vivo anticancer activity of
AZD3965 either alone (monotherapy) or with other interventions (combination
therapy). Study search was performed in nine different databases using the keywords "
AZD3965 in vivo" as search terms. The results show that
AZD3965 successfully decreased
tumor growth and promoted intracellular
lactate accumulation, which confirmed its effectiveness, especially in combined
therapy. These results support the setup of clinical trials, but other important findings, namely
AZD3965 enhanced activity when given in combination with other
therapies, or MCT4-induced treatment resistance, should be further considered in the clinical trial design to improve
therapy response.