Abstract | BACKGROUND: Abnormal expression of long noncoding RNAs (lncRNAs) has been reported in the acute stage of acute ischemic stroke (AIS). This study aimed to explore differential lncRNA expression in the subpopulations of peripheral blood mononuclear cells (PBMCs) from AIS patients and further evaluate its underlying mechanisms in stroke-induced immunosuppression. METHODS: We reanalyzed lncRNA microarray data and investigated abnormally expressed lncRNAs in the subpopulations of PBMCs by magnetic cell sorting and real-time quantitative PCR. The potential mechanism of small nucleolar RNA host gene 15 (SNHG15) was explored through in vitro and in vivo approaches. RESULTS: CONCLUSIONS: This study identified SNHG15 as a negative regulator of inflammation in stroke-induced immunosuppression, suggesting it as a novel biomarker and therapeutic target in stroke-associated infection. Trial registration ClinicalTrials.gov NCT04175691. Registered November 25, 2019, https://www.clinicaltrials.gov/ct2/show/NCT04175691 .
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Authors | Huiling Sun, Shuo Li, Zhaohan Xu, Chengfang Liu, Pengyu Gong, Qiwen Deng, Fuling Yan |
Journal | Journal of neuroinflammation
(J Neuroinflammation)
Vol. 19
Issue 1
Pg. 1
(Jan 03 2022)
ISSN: 1742-2094 [Electronic] England |
PMID | 34980176
(Publication Type: Journal Article)
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Copyright | © 2021. The Author(s). |
Chemical References |
- Cytokines
- Lipopolysaccharides
- RNA, Long Noncoding
- TNF Receptor-Associated Factor 2
- Interleukin-4
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Topics |
- Cytokines
(metabolism)
- Humans
- Immune Tolerance
- Inflammation
(immunology, metabolism)
- Interleukin-4
(pharmacology)
- Lipopolysaccharides
(pharmacology)
- Macrophages
(drug effects, metabolism)
- Monocytes
(drug effects, metabolism)
- RNA, Long Noncoding
(genetics, metabolism)
- Stroke
(immunology, metabolism)
- TNF Receptor-Associated Factor 2
(genetics, metabolism)
- Ubiquitination
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