A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes.
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| Abstract |
Hematophagous arthropods, such as mosquitoes, naturally carry and transmit hundreds of arboviruses to humans. Blood meal is a predominant physical interface that shapes cross-species communications among humans, bloodsuckers, and arboviruses. Here, we identify a human-blood-derived microRNA, hsa-miR-150-5p, that interferes with a mosquito antiviral system to facilitate flavivirus infection and transmission. hsa-miR-150-5p is acquired with a blood meal into the mosquito hemocoel and persists for a prolonged time there. The agomir of hsa-miR-150-5p enhances, whereas the antagomir represses flaviviral infection in mosquitoes and transmission from mice to mosquitoes. Mechanistic studies indicate that hsa-miR-150-5p hijacks the mosquito Argonaute-1-mediated RNA interference system to suppress the expression of some chymotrypsins with potent virucidal activity. Mosquito chymotrypsins are essential for resisting systemic flavivirus infection in hemocoel tissues. Chymotrypsin homologs potentially targeted by miR-150-5p are also found in other hematophagous arthropods, demonstrating a conserved miR-150-5p-mediated cross-species RNAi mechanism that might determine flaviviral transmissibility in nature.
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| Authors | Yibin Zhu, Chi Zhang, Liming Zhang, Yun Yang, Xi Yu, Jinglin Wang, Qiyong Liu, Penghua Wang, Gong Cheng |
| Journal | Cell reports (Cell Rep) Vol. 37 Issue 11 Pg. 110091 (12 14 2021) ISSN: 2211-1247 [Electronic] United States |
| PMID | 34910910 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved. |
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