Effect of methanolic extract of Citrus limetta peel on cellular and humoral immune response in mice.

Abstract

Citrus limetta is well known for its anti-inflammatory, antimicrobial, antifungal, antidiabetic and antioxidant properties. Methanolic extract of Citrus limetta (MECL) was used to assess cellular and humoral immune responses in mice by carrying out cyclophosphamide-induced neutropenia, delayed-type hypersensitivity (DTH), carbon clearance assay, haemagglutination assay (HA) and mice lethality assay. Methanolic extract of Citrus limetta peel was administered orally to mice in two doses 200mg/kg and 400mg/kg.The extract treated groups showed improvement in neutropenia induced by cyclophosphamide and improvement in the WBC profile. Skin thickness was significantly (P<0.05) higher in 200mg/kg and 400mg/kg groups in comparison to control in DTH. The phagocytic index was significantly (P<0.05) more in 400mg/kg group in carbon clearance assay. Mice were vaccinated with hemorrhagic septicemia vaccine before challenge with Pasteurella multocida for mice lethality test. Percentage mortality was decreased in 400mg/kg treated group in comparison to negative control Antibody titre response to sheep red blood cells was significantly (P<0.05) higher with dose 400mg/kg in HA. Results suggested the effectiveness of the methanolic extract of Citrus limetta as an immunostimulating agent.

• Authors: Sarwat Shaheen, Aqeel Javeed, Adeel Sattar, Aammir Ghafoor, Shahzada Khurram Syed
• Journal: Pakistan journal of pharmaceutical sciences (Pak J Pharm Sci) Vol. 34 Issue 5(Supplementary) Pg. 1861-1866 (Sep 2021) ISSN: 1011-601X [Print] Pakistan
• PMID: 34836851 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Antibodies, Bacterial
• Plant Extracts
• Solvents
• Carbon
• Cyclophosphamide
• Methanol

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antibodies, Bacterial (analysis)
• Carbon (metabolism)
• Citrus (chemistry)
• Cyclophosphamide
• Fruit (chemistry)
• Immunity, Cellular (drug effects)
• Immunity, Humoral (drug effects)
• Leukocyte Count
• Methanol
• Mice
• Neutropenia (chemically induced, drug therapy)
• Pasteurella Infections (immunology, prevention & control)
• Pasteurella multocida (immunology)
• Phagocytosis (drug effects)
• Plant Extracts (pharmacology)
• Sheep
• Skin (drug effects)
• Solvents