Abstract | BACKGROUND: OBJECTIVE: In this study, we will examine the effectiveness of combined treatment of ALA- PDT and HL in cSCC as well as its underlying mechanism. METHODS: The human epidermoid carcinoma cell line SCL-1 was treated with ALA- PDT or/ and HL, and cell viability, cell migration, ROS production, apoptosis were evaluated by CCK-8, colony formation, scratch assay, DCFH-DA probe, flow cytometry, respectively. The protein expression of NRF2 signaling was examined by western blot. RESULTS: HL strengthened ALA- PDT's inhibition of SCL-1 cell viability, migration, as well as NRF2 related β- catenin, p-Erk1/2, p-Akt and p-S6K1 expression. Overexpression of NRF2 conferred resistance to co-treatment's effects on c-Myc, Cyclin D1, Bcl-2, as well as cell proliferation. HL also strengthened ALA- PDT's inhibition of tumor volume in cSCC mouse model and elevated ROS generation of ALA- PDT. CONCLUSION: HL enhances the anti- tumor effect of ALA- PDT in vitro and in vivo. HL has the potential to enhance the anti- tumor effect of ALA- PDT in cSCC via inhibiting NRF2 signaling.
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Authors | Ting Lv, Jianhua Huang, Minfeng Wu, Hongwei Wang, Qingyu Zeng, Xiuli Wang |
Journal | Photodiagnosis and photodynamic therapy
(Photodiagnosis Photodyn Ther)
Vol. 37
Pg. 102572
(Mar 2022)
ISSN: 1873-1597 [Electronic] Netherlands |
PMID | 34628069
(Publication Type: Journal Article)
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Copyright | Copyright © 2021. Published by Elsevier B.V. |
Chemical References |
- NF-E2-Related Factor 2
- Photosensitizing Agents
- Piperidines
- Quinazolinones
- Aminolevulinic Acid
- halofuginone
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Topics |
- Aminolevulinic Acid
(pharmacology, therapeutic use)
- Animals
- Apoptosis
- Carcinoma, Squamous Cell
(drug therapy)
- Cell Line, Tumor
- Mice
- NF-E2-Related Factor 2
(pharmacology, therapeutic use)
- Photochemotherapy
(methods)
- Photosensitizing Agents
(pharmacology, therapeutic use)
- Piperidines
- Quinazolinones
- Skin Neoplasms
(pathology)
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