Abstract | BACKGROUND: METHODS: The level of EMMPRIN expression was evaluated using reverse transcriptase polymerase chain reaction (RT-PCR) in 6 tumor-derived osteosarcoma cell lines and compared with that in normal osteoblasts. To study the prognostic significance of EMMPRIN expression, immunohistochemistry was carried out in prechemotherapy biopsies of 54 patients. siRNA knockdown of EMMPRIN in SaOS-2 cells was conducted to explore the role of EMMPRIN. To study the role of EMMPRIN in tumor-stromal interaction in MMP production and invasion, co-culture of SaOS-2 cells with osteoblasts and fibroblasts was performed. Osteosarcoma 143B cells were injected into the tail vein of BALB/c mice and lung metastasis was analyzed. RESULTS: EMMRIN mRNA expression was significantly higher in 5 of 6 (83%) tumor-derived cells than in MG63 cells. 90% of specimens (50/54) stained positive for EMMPRIN by immunohistochemistry, and higher expression of EMMPRIN was associated with shorter metastasis-free survival (p = 0.023). Co-culture of SaOS-2 with osteoblasts resulted in increased production of pro-MMP2 and VEGF expression, which was inhibited by EMMPRIN-targeting siRNA. siRNA knockdown of EMMPRIN resulted in decreased invasion. EMMPRIN shRNA-transfected 143B cells showed decreased lung metastasis in vivo. CONCLUSIONS:
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Authors | Han-Soo Kim, Ha Jeong Kim, Mi Ra Lee, Ilkyu Han |
Journal | BMC cancer
(BMC Cancer)
Vol. 21
Issue 1
Pg. 1059
(Sep 26 2021)
ISSN: 1471-2407 [Electronic] England |
PMID | 34565336
(Publication Type: Journal Article)
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Copyright | © 2021. The Author(s). |
Chemical References |
- RNA, Messenger
- RNA, Small Interfering
- Vascular Endothelial Growth Factors
- Basigin
- Matrix Metalloproteinase 2
- MMP1 protein, human
- Matrix Metalloproteinase 1
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Topics |
- Animals
- Basigin
(antagonists & inhibitors, metabolism)
- Bone Neoplasms
(metabolism, pathology)
- Cell Line, Tumor
- Coculture Techniques
- Disease Progression
- Humans
- Immunohistochemistry
- Lung Neoplasms
(secondary)
- Matrix Metalloproteinase 1
(biosynthesis)
- Matrix Metalloproteinase 2
(biosynthesis)
- Mice
- Mice, Inbred BALB C
- Neoplasm Invasiveness
- Osteoblasts
(metabolism)
- Osteosarcoma
(metabolism, pathology, secondary)
- Progression-Free Survival
- RNA, Messenger
(metabolism)
- RNA, Small Interfering
(pharmacology)
- Vascular Endothelial Growth Factors
(metabolism)
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