Abstract | BACKGROUND: RESULTS: The synthesized Ag-MOF particles had uniform size and shape of radiating corona. The mean nanoparticle size and zeta potential of PLT@Ag-MOF-Vanc were 148 nm and - 25.6 mV, respectively. The encapsulation efficiency (EE) and loading efficiency (LE) of vancomycin were 81.0 and 64.7 %, respectively. PLT@Ag-MOF-Vanc was shown to be a pH-responsive nano-drug delivery system with good biocompatibility. Ag-MOF had a good inhibitory effect on the growth of three common clinical strains (Escherichia coli, Pseudomonas aeruginosa, and S. aureus). PLT@Ag-MOF-Vanc showed better antibacterial activity against common clinical strains in vitro than free vancomycin. PLT@Ag-MOF-Vanc killed MRSA through multiple approaches, including interfering with the metabolism of bacteria, catalyzing reactive oxygen species production, destroying the integrity of cell membrane, and inhibiting biofilm formation. Due to the encapsulation of the platelet membrane, PLT@Ag-MOF-Vanc can bind to the surface of the MRSA bacteria and the sites of MRSA infection. PLT@Ag-MOF-Vanc had a good anti-infective effect in mouse MRSA pneumonia model, which was significantly superior to free vancomycin, and has no obvious toxicity. CONCLUSIONS: PLT@Ag-MOF-Vanc is a novel effective targeted drug delivery system, which is expected to be used safely in anti-infective therapy of MRSA.
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Authors | Rong Huang, Guang-Qing Cai, Jian Li, Xi-Sheng Li, Hai-Ting Liu, Xue-Ling Shang, Jian-Dang Zhou, Xin-Min Nie, Rong Gui |
Journal | Journal of nanobiotechnology
(J Nanobiotechnology)
Vol. 19
Issue 1
Pg. 229
(Aug 04 2021)
ISSN: 1477-3155 [Electronic] England |
PMID | 34348721
(Publication Type: Journal Article)
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Copyright | © 2021. The Author(s). |
Chemical References |
- Anti-Bacterial Agents
- Drug Carriers
- Metal-Organic Frameworks
- Nanoparticle Drug Delivery System
- Vancomycin
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Topics |
- Animals
- Anti-Bacterial Agents
(pharmacology)
- Disease Models, Animal
- Drug Carriers
(pharmacology)
- Escherichia coli
(drug effects)
- Male
- Metal-Organic Frameworks
(pharmacology)
- Methicillin-Resistant Staphylococcus aureus
(drug effects)
- Mice
- Microbial Sensitivity Tests
- Nanoparticle Drug Delivery System
(pharmacology)
- Nanoparticles
- Pseudomonas aeruginosa
(drug effects)
- RAW 264.7 Cells
- Staphylococcus aureus
(drug effects)
- Vancomycin
(pharmacology)
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