Stress-related changes in the gut microbiome after trauma.

Abstract

BACKGROUND:

The gut microbiome protects the host from infection by promoting epithelial integrity and providing basal immunologic stimulation. Disruption of this delicate ecosystem is linked to morbidity and mortality among critically ill patients, but the impact of traumatic injury on the gut microbiome is poorly understood. This study sought to identify alterations in gut microbiota following trauma and persistent stress in rodents without confounding antibiotics.

METHODS:

Male Sprague-Dawley rats aged 9 weeks to 11 weeks were randomized to naive, lung contusion with hemorrhagic shock (LCHS), and LCHS plus either 7 (LCHS/CS 7/7) or 14 days (LCHS/CS 14) of restraint cylinder stress for 2 hours daily. Stool was collected on Days 0, 3, 7, and 14 for bacterial whole genome DNA isolation. Alpha diversity, or the number and relative abundance of unique bacterial species within each cohort, was assessed using Chao1 indices. Beta diversity, or the measure of differences in biodiversity across cohorts, was assessed by principle coordinate analysis. False discovery rate correction was applied to all statistical analyses and corrected for cohousing effects.

RESULTS:

Rodent groups subject to restraint stress demonstrated a progressive increase in alpha diversity over time. These microbiota changes resolved after cessation of stress (LCHS/CS 7/7) but continued to increase among rats subjected to ongoing stress (LCHS/CS 14). The LCHS/CS 7/7 also demonstrated reductions in class Actinobacteria and increased abundance of the genus Bacteroides by Day 7, which resolved by Day 14. Increased abundance of Bacteroides was also noted in the LCHS/CS 14 cohort, suggesting the role of chronic stress in its destabilization.

CONCLUSION:

This study points to persistent stress as a potential source of the destabilization of microbial diversity seen after trauma. This lack of microbiota stability could be associated with worse long-term outcomes in critically ill trauma patients. Further studies are warranted to elucidate mechanistic pathways and potential therapeutic modalities.

• Authors: Lauren S Kelly, Camille G Apple, Raad Gharaibeh, Erick E Pons, Chase W Thompson, Kolenkode B Kannan, Dijoia B Darden, Philip A Efron, Ryan M Thomas, Alicia M Mohr
• Journal: The journal of trauma and acute care surgery (J Trauma Acute Care Surg) Vol. 91 Issue 1 Pg. 192-199 (07 01 2021) ISSN: 2163-0763 [Electronic] United States
• PMID: 34144563 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.

Chemical References

• DNA, Bacterial

Topics

• Animals
• Contusions (pathology)
• DNA, Bacterial (genetics)
• Feces (microbiology)
• Gastrointestinal Microbiome
• Lung Injury (complications, pathology)
• Male
• Rats
• Rats, Sprague-Dawley
• Restraint, Physical
• Shock, Hemorrhagic (microbiology)
• Stress, Physiological