The superposition anti-viral activity of porcine tri-subtype interferon expressed by Saccharomyces cerevisiae.

Abstract

Interferon (IFN)-mediated antiviral responses are central to host defense against viral infection. Porcine viral infection has emerged as a serious hazard for the pig industry. The construction of an engineered Saccharomyces cerevisiae strain that efficiently produces porcine IFN has demonstrated several advantages. It can be easily fed to pigs, which helps in reducing antibiotic residues in pork and improve meat quality. In this study, the stable expression of several porcine IFN molecules (pIFN-α1, pIFN-β, pIFN-λ1, pIFN-λ1-β, pIFN-λ1-β-α1) were determined using an engineered S. cerevisiae system. With the YeastFab assembly method, the complete transcriptional units containing promoter (GPD), secretory peptide (α-mating factor), target gene (IFN) and terminator (ADH1) were successfully constructed using the characteristics of type II restriction endonuclease, and then integrated into the chromosomes Ⅳ and XVI of ST1814 yeast host strain, respectively. The expression kinetics of recombinant pIFNs were further analyzed. Synergism in the expression level of IFN receptor, antiviral protein, and viral loading was observed in viral-cell infection model treated with different porcine IFN subtypes. The porcine reproductive and respiratory syndrome viral load and antibody titer in serum decreased significantly after oral administration of IFN expression yeast fermentation broth. These findings indicate the potential efficacy of multi-valent pIFNs expressing S. cerevisiae as a potent feed material to prevent viral infections of pigs.

• Authors: Hui Zhai, Jingxuan Shi, Ruiqi Sun, Zheng Tan, Umm E Swaiba, Wanqing Li, Lilin Zhang, Lei Zhang, Yanyu Guo, Jinhai Huang
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 259 Pg. 109150 (Aug 2021) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 34144506 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• Culture Media
• Interferons

Topics

• Animals
• Antiviral Agents (administration & dosage, immunology)
• Cell Line
• Culture Media (analysis)
• Fermentation
• Gene Expression
• Interferons (administration & dosage, classification, genetics, immunology)
• Porcine Reproductive and Respiratory Syndrome (prevention & control)
• Porcine respiratory and reproductive syndrome virus (immunology)
• Saccharomyces cerevisiae (genetics, growth & development, immunology)
• Swine (immunology)
• Swine Diseases (immunology, prevention & control, virology)
• Virus Diseases (immunology, prevention & control, veterinary)
• Virus Replication