Exosomes from human umbilical cord mesenchymal stem cells (HUCMSCs) containing
microRNAs (
miRNAs) have been underscored as possible therapeutic options for
cancers. Hence, our goal here was to investigate the relevance of miR-320a-containing exosomes from HUCMSCs to
lung cancer. First, H1299 and H460 cells were co-cultured with the exosomes overexpressing miR-320a from HUCMSCs. The data displayed that HUCMSCs-secreted exosomes expressing miR-320a exerted anti-
tumor effects in vitro and in vivo. Online analysis available at TargetScan database revealed that miR-320a bound to sex-determining region Y-box 4 (SOX4), and the
luciferase reporter gene assay clarified this targeting relationship. Next, a β-
catenin-specific agonist
WAY-262611 was delivered into the H1299 and H460 cells to assess the effects of the Wnt/β-
catenin pathway on
lung cancer cellular processes. The results demonstrated that
WAY-262611 potentiated
lung cancer cell viability, invasion, and migration, but inhibited cell apoptosis. Altogether, exosomes carrying miR-320a from HUCMSCs might suppress
lung cancer cell growth via the SOX4/Wnt/β-
catenin axis, which highpoints the potency of exosomes expressing miR-320a as a possible therapeutic option for
lung cancer treatment.